IS IT A VIRUS OR IS IT GRAPHENE TOXICITY?

& HOW CAN ANYONE MANDATE OR ENFORCE THESE TOXIC TESTS, MASKS AND INJECTIONS?

PLEASE TAKE A VERY CLOSE LOOK:

GRAPHENE IS A BIOCIDE:

https://www.carbon-waters.com/graphene-a-superb-biocidal-agent/ https://www.versarien.com/files/5916/3707/5814/Development_of_graphene_and_nanoparticle-based_anti-microbials.pdf & https://www.graphene-info.com/how-can-graphene-assist-war-coronavirus & https://www.globenewswire.com/news-release/2021/03/08/2188871/0/en/Ceylon-Graphite-Files-Patent-for-Newly-Developed-Biocidal-Nanocomposite-Surface-Coating-Material.html

AND THE BIOCIDE WAS SPRAYED AND WIDELY USED TO "PREVENT THE VIRUS."

https://www.naturalnews.com/2022-06-09-spanish-government-admits-spraying-chemtrails-on-citizens.html

I. GENERAL PROVISIONS

MINISTRY OF HEALTH

4492 Order SND/351/2020, of April 16, authorizing the NBC Units of the Armed Forces and the Military Emergency Unit to use biocides authorized by the Ministry of Health in the disinfection to deal with the health crisis caused by COVID-19.

Royal Decree 463/2020, of March 14, declaring a state of alarm for the management of the crisis situation caused by COVID-19, contemplates a series of measures aimed at protecting the welfare, health and safety of the public and containing the progression of the disease and strengthen the public health system.

Article 4.2.d) of the aforementioned Royal Decree 463/2020, of March 14, determines that, for the exercise of the functions foreseen in the same and under the superior direction of the President of the Government, the Minister of Health shall have the status of delegated competent authority, both in his own area of responsibility and in the other areas that do not fall under the areas which do not fall within the specific sphere of competence of the other heads of the departments designated as competent authorities for the purposes of the effects of the aforementioned Royal Decree.

Specifically, in accordance with the provisions of article 4.3 of Royal Decree 463/2020, of March 14th, the Minister of Health is empowered to issue the orders, resolutions, provisions and instructions which, within his scope of action as a delegated authority, may be necessary to guarantee the provision of all services, ordinary or extraordinary, in order to protect persons, goods and places, by means of the adoption of any of the measures foreseen in provided for in Article eleven of Organic Law 4/1981, of June 1, 1981, on states of alarm, exception and siege.

For the effective fulfillment of these measures, the competent delegated authorities may require the authorities may require the action of the Armed Forces, in accordance with the provisions of article 15.3 of the

Article 15.3 of Organic Law 5/2005, of November 17, 2005, on National Defense.

In the field of the containment of the spread of the coronavirus, special attention is required to disinfection of facilities such as residential social centers, hospitals and other health centers, penitentiary establishments, traffic management centers and transport hubs.

The Armed Forces are carrying out these tasks as one of their priority tasks.

The Ministry of Health has been publishing and updating the list of biocides to be used against the new coronavirus, which are authorized and registered in Spain in accordance with the UNE-EN 14476, which evaluates the virucidal capacity of antiseptics and chemical disinfectants. In particular, due to their special efficacy, some biocides are specified as follows biocides established in the main group 1 of article 1.1 of Royal Decree 830/2010, of 25 June, which establishes the regulations governing the training to carry out treatments with biocides.

Among the most effective disinfection techniques are the use of aerial means, since, through aerial means because through them, with nebulization, thermonebulization and micronebulization techniques, all surfaces can be reached quickly, avoiding the need to depend on manual application, which is slower and sometimes does not reach all surfaces due to obstacles that prevent them from being reached.

The NBC defense units of the Armed Forces and the Military Emergency Unit (UME) have the following at their disposal personnel, materials and procedures.

OFFICIAL STATE BULLETIN

No. 107 Friday, April 17, 2020 Sec. I. Page 29198 cve: BOE-A-2020-4492

Verifiable at https://www.boe.es sufficient training to carry out aerial disinfections, as they are operations that are regularly performed regularly, except that instead of using biocidal products, they use other decontaminating chemical products.

In view of the above, and in order to improve and speed up disinfection operations in all types of facilities that the personnel of the Armed Forces have been carrying out, it is considered convenient to authorize

the Armed Forces, it is considered convenient to authorize, on an exceptional basis and for the duration of state of alarm, to the NBC Defense Units of the Armed Forces and the UME, the use of the disinfectants and biocides of the main group 1 described in article 1.1 of Royal Decree 830/2010, of June 25, 2010, which establishes the regulations governing the training to carry out treatments with biocidal products, which have been authorized and indicated as effective for the control of the COVID-19 pandemic.

By virtue, thereof, in accordance with the powers conferred by Article 4.3 of Royal Decree 463/2020, of March 14th, I hereby resolve:

First.

Authorization to the NBQ Units of the Armed Forces and the Military Emergency Unit to use biocides authorized by the Ministry of Health.

The NBC Units of the Armed Forces and the Military Emergency Unit are authorized to use biocides authorized by the Ministry of Health.

Emergency, within the general actions of disinfection of spaces, both public and private, that on the occasion of the state of alarm situation have been carried out by the mentioned units, to use those biocides of the main group 1, described in article 1.1 of Royal Decree 830/2010, of June 25, 2010, which establishes the regulations governing the training to carry out treatments with biocides, which are authorized and listed by the Ministry of Health as effective in the fight against COVID-19.

Likewise, the units mentioned in the previous paragraph are authorized to use aerial disinfection procedures of aerial disinfection procedures, through the techniques of nebulization, thermonebulization and micronebulization, for the execution of the aforementioned disinfection tasks.

Second.

Validity.

The measures foreseen in the present order will be applicable during the validity of the state of alarm declared by Royal Decree 463/2020, of March 14, and its possible extensions.

Three.

Effects.

This order shall take effect on the same day of its publication in the "Official Gazette of the State".

Fourth.

Appeals.

Against the present order, which puts an end to administrative proceedings, an administrative appeal may be filed within a period of two months as of the day following the date of its publication, before the Contentious-Administrative Chamber of the Supreme Court, in accordance with the provisions of Article 12 of Law 29/1998, of July 13, 1998, regulating the Contentious-Administrative Jurisdiction.

Madrid, April 16, 2020.-The Minister of Health, Salvador Illa Roca.

OFFICIAL STATE BULLETIN

No. 107 Friday, April 17, 2020 Sec. I. Page 29199 cv -

IMPACT ON HEALTH:

THE FIRST THING THAT SHOULD BE INVESTIGATED IS WHETHER IT IS A VIRUS OR GRAPHENE TOXICITY.

However, 4 of 9 mice treated with 0.4 mg per mouse died (1/3 in the 1-day group, 1/3 in the 7-day group and 2/3 in the 30-day group). All deaths occurred 1–7 days after injection of the GRAPHENE OXIDE GO. The deaths were generally preceded by lethargy, inactivity, and body-weight losses. Histopathology of lung tissues showed that major airways of four mice were mechanically blocked by the GO conglomeration, which led to suffocation in 15% of the GO-exposed mice, and was not evidence of pulmonary toxicity of GO.

We also investigated the effects of GRAPHENE OXIDE GO on organs of mice. We learn from the pathology and light micrograph that the GO accumulations were primarily in the lungs, liver, and spleen. There were obvious chronic toxicity responses occurring in the lungs and liver after tail vein injection. Histopathological analysis revealed that pulmonary exposures to GO produced a dose-dependent lung inflammatory response characterized by neutrophils and foamy alveolar macrophage accumulation. Figure Figure66 showed the light micrograph of lung tissues from mice exposed to different doses of GO for 7 days, clearly showed that the treated mice exhibited a dose-dependent series of granulomas. With the increase in GO dose, the toxicity reaction of the lung of mice becomes more and more severe. For example, GO induced dose-dependent epithelioid granulomas and, in some cases, interstitial inflammation in the mice. Large amount of inflammation cells was infiltrated in lung alveolus interstitium; the alveolar septa became thicker and some lung alveoli were cracked.

According to our results, we suggest the possible mechanism of GO's cytotoxicity as follows: GO in medium attach to the surface of human cells, providing a stimuli signal to the cells. The signal is transduced inside the cells and the nucleus, leading to down-regulation of adhesion-associated genes and corresponding adhesive proteins, resulting in decrease in cell adhesion and causing cells to detach, float, and shrink in size. At the same time, GO enters into cytoplasm by endocytosis pathway, mainly located in the lysosomes, mitochondrion, endoplasm and cell nucleus, may disturb the course of cell energy metabolism and gene transcription and translation, and finally result in cell apoptosis or death.

In conclusion, our primary studies have indicated that GO could produce cytotoxicity in dose- and time-dependent means, and can enter into cytoplasm and nucleus, decreasing cell adhesion, inducing cell floating and apoptosis. GO can enter into lung tissues and stop there and induce lung inflammation and subsequent granulomas highly dependent on injected dose. Exposures to GO may induce severe cytotoxicity and lung diseases. It should be the first report. Although GO has been investigated for biomedical applications such as cell imaging and drug delivery, because of GO's long-term stay in kidney and being very difficult to be cleaned by kidney, therefore, GO may not own good application prospect in human body. (!!!!!!!!)

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212228/ Biocompatibility of Graphene Oxide

Inhaled carbon nanotubes can suppress the immune system by affecting the function of T cells, a type of white blood cell that organizes the immune system to fight infections.

https://www.ohsrep.org.au/nanotechnology_-_a_new_hazard

Two recent studies give us a less than rosy angle. In the first, a team of biologists, engineers and material scientists at Brown University examined graphene’s potential toxicity in human cells. They found that the jagged edges of graphene nanoparticles, super sharp and super strong, easily pierced through cell membranes in human lung, skin and immune cells, suggesting the potential to do serious damage in humans and other animals. (!!!!!!!!)

https://newatlas.com/graphene-bad-for-environment-toxic-for-humans/31851/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468375/ Graphene and the Immune System: A Romance of Many Dimensions

These indirect pathways for damage ultimately contribute to DNA lesions, double strand breaks, aneuploidy and oxidative DNA damage. Secondary genotoxicity is typically observed in vivo and is the result of DNA damage induced through a (sub)-chronic immune response, involving immune cell activation and recruitment, heightened inflammatory activity and subsequent oxidative stress, promoting genotoxicity in the surrounding epithelial cells.

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-021-00769-9

However, the intolerance of the immune system to graphene nanomaterials, however low, may in consequence make it impossible to use them in medicine.

https://pubmed.ncbi.nlm.nih.gov/26502273/ The Molecular Influence of Graphene and Graphene Oxide on the Immune System Under In Vitro and In Vivo Conditions

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-021-00769-9 Few-layer graphene induces both primary and secondary genotoxicity in epithelial barrier models in vitro | Journal of Nanobiotechnology

Mechanisms of graphene cytotoxicity

The main mechanism of cytotoxicity is the generation of ROS and thus oxidative stress, as has been reported. GNMs could induce intercellular ROS generation and subsequently interrupt the dynamic balance of ROS, and then cause consequential damage to DNA and proteins, and eventually apoptosis or necrosis [

Methods for in vivo toxicity assessment

It has been demonstrated that nanomaterials can invade into the animal body through inhalation, skin contact and other deliberate administrations, including oral feeding and various injections.

Therefore, the in vivo toxicity of nanoparticles is far beyond necessary to be studied, given that all nanoparticles tend to be toxic due to chronic exposure and consequent accumulation in certain organs. Furthermore, as graphene and its derivatives have been proven to interact with biomolecules and occasionally be harmful to cells, the assessments of in vivo toxicity are urgently required to better understand the map of graphene toxicity. Some efforts have been made in the field by studying the blood properties and thrombus, PKs and pathological changes. In this section we take several studies on the in vivo toxicity of GNMs as examples, and discuss the toxicity evaluation strategies in detail, Blood compatibility Nanomaterials used for biomedical applications should be stable and compatible with blood, so it can retain the engineered functions in vivo.

Otherwise, they tend to aggregate, adsorb plasma proteins and platelet due to their high surface energy as well as large surface area, and consequently trigger coagulation and clot formation, as well as activate complement cascades.

As a result, understanding the blood contact property of GNMs is the priority before their practical applications. In a study of Dash and coworkers, Swiss male mice of 2–3 months were intravenously (iv.) administered with GO or RGO at the dose of 0.25 mg/kg for the study of GO-induced pulmonary thromboembolism. The authors found a large number of platelet rich thrombi in lung vessels; also, they conducted several in vitro assays to probe the mechanism of GO induced thrombus formation. According to the measurements of PAC-1 binding, F-actin content, LDH leakage, calcium and ROS generation, GO activating platelets was attributed to the release of intracellular free calcium from cytosolic stores and activation of nonreceptor protein tyrosine kinases of the Src family in platelets. In another work from the same group, flow cytometry, in which side-scattering signals reflect internal complexities of particles and reveal the extent of GO–cell interaction, was employed to verify the inert surface of GO-NH2 (amine-modified graphene) for platelet adhesion. Lee and colleagues utilized anti-FXa activity to evaluate the blood compatibility of GO and GO-heparin, since FXa regulates thrombin generation and thus is critical to the blood coagulation.

https://www.sciencedirect.com/science/article/pii/S2352940718302853 Investigation into the toxic effects of graphene nanopores on lung cancer cells and biological tissues

However, inhalation of graphene structures is believed to be a risk factor for cardiorespiratory disease. For example, inhaled graphene nanoplatelets can be transported deep within the distal regions of the lungs and trigger chronic inflammation in the respiratory tract. It is generally thought that the placenta, lung, gastrointestinal tract and skin act as major barriers for many nanostructures entry into living organisms. Indeed, a recent study on mice demonstrated that intratracheally delivered few-layered graphene was mainly retained in the lung, with 47% remaining after 4 weeks and this resulted dose-dependent acute lung injury and pulmonary oedema. An in vitro study of the effects of graphene and graphene oxide on human skin HaCaT keratinocytes demonstrated that oxidized graphene was the most cytotoxic, inducing mitochondrial and plasma-membrane damage, and suggesting low cytotoxic effects at the skin level. Reduced graphene oxide is more toxic than graphene oxide as evidenced by many studies reported recently. This is primarily due to its sharp edges and structural morphology. In contrast to the typically soluble nanoparticles examined in conventional toxicology investigations, graphene nanostructures have different shapes and surface areas, and which in turn can significantly influence their diffusion, dispersion, aggregation and agglomeration in plasma. Importantly, these “tunable” characteristics of graphene account for the varying toxic outcomes on the tissues. In vivo, following toxicity testing of graphene, post-mortem histological examinations of liver alterations have revealed hypertrophy of hepatocytes, necrosis and inflammatory cell infiltration in liver and kidney tissues.

Antioxidants act as a defense system to reinstate the cellular redox balance when oxidative stress is generated as a result of excess production of reactive oxygen species.

Disruption of this critical balance in the presence of excessive reactive oxygen species triggers the activation and promotion of a pro-inflammatory cascade, which in turn may cause mitochondrial release of proapoptotic factors potentially leading to cell death. Hepatocytes are key targets for reactive oxygen species damage, and therefore liver function and biomarkers of oxidative stresses should be investigated with great care.

Specifically, vacuolation, dilation of central vein and hemorrhage, vacuolation and dilation of central vein, damage of vacuolation, hemorrhage and degeneration of central vein, dilation of epithelial lining and hydropic degeneration oedema were observed in liver tissue. Kidney tissue of the treated groups showed acute vacuolization, dilation of epithelial lining, vacuolation and nucleus degeneration, nucleus damage, necrosis and epithelial degeneration.

Heart tissue showed chemodectoma, toxic myocarditis, reddish brown atrophy; yellowish brown pigments suggesting lipofuscin granules as remnants of cell organelles and cytoplasmic material.

The brain showed effects of secondary carcinoma, olegodendrocytoma small thin walled blood vessel and crytococcosis. Testicular tissue of treated groups showed spermatogenesis and vacuolation, dilation of germinal layer, degeneration of secondary spermatocytes, damage to the germinal layer and vacuolation. The lung showed damage of vacuolation, degeneration of central vein, inflammation, haemorrhage, d-shaped cells structure, hemosidophroages and lesion.

Indeed, 3D porous graphene frameworks have shown various effects from acute lethally to sub lethal toxic effects including histological, and oxidative stress responses and, after inhalation exposure in rats, graphene has been found to accumulate in the lung, leading to phagocytosis.

In this study, the first sign of toxicity recorded for the rats given an intraperitoneal injection of GNPs was an observed decrease in body weight at the higher dose. Toxic effects of GNPs on CBC were not observed although there was a slight (6%) reduction in platelet numbers in the 15 mg/kg group. The increased activities of AST, ALT, AMP and creatinine observed after 27 days are indicators of liver and kidney toxicity respectively and appeared in the rats receiving both single and multiple doses of GNPs, compared to control groups. Severe organ damage can increase the activities of ALT and AST and enhanced activity of both are observed when disease processes affect liver cell integrity. Importantly, increased serum ALT activity reflects specific hepatocellular injury. Histopathological alterations were also evident in the liver, where GNPs induced dose- and time-dependent histological alterations of the liver tissues, including congestion, prominent vasodilatation, and vacuolization.

https://pubs.rsc.org/en/content/articlelanding/2015/nr/c5nr01839k Protein corona mitigates the cytotoxicity of graphene oxide by reducing its physical interaction with cell membrane

However, the underlying molecular mechanisms of how the so-called “protein corona” formed in serum medium affects nanoparticles’ biological responses are still largely unresolved. Thus, it is critical to understand how absorbed proteins on the surfaces of nanoparticles alter their biological effects.

Is Covid a "Wuhan" virus with some bizarre symptoms? Or is it a pandemic of graphene toxicity?

http://web.archive.org/web/20200804115842/https://www.sqnewmaterials.com/biomass-graphene-disposable-medical-face-mask/57701908.html

https://www.country94.ca/2021/04/04/graphene-containing-masks-recalled/

“A panel of graphene-based materials, including few-layer graphene, graphene, graphene oxide, and reduced GO did not induce irritation unless they were prepared with irritant surfactants such as sodium dodecyl sulfate and sodium dodecyl-benzenesulfonate. Nanoparticles could also reach the lungs if nanoparticles are not firmly embedded in the face mask material. Acute effects in humans arising from inhalation of silver nanoparticles include lung failure, increased heart rate and decreased arterial blood oxygen pressure.”

“Passage of inhaled nanoparticles into the bloodstream was demonstrated in one human study (Nemmar et al 2002), but two other similar studies have failed to show such a translocation. Another potential route of translocation of inhaled nanoparticles is the olfactory nerve in the nose leading to the olfactory bulb of the brain. 13C nanoparticles with a size about 35 nm were detected in the brain olfactory bulb after inhalation exposure. The route of brain entry was suggested to be by migration along the olfactory nerve into the olfactory bulb of the brain after deposition on the olfactory mucosa in the nasal region (Oberdörster E et al 2004).”

https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-016-0120-1 Biodistribution and toxicity of radio-labeled few layer graphene in mice after intratracheal instillation

When considering the potential human health risks of nanoparticles, inhalation is thought to be the exposure route of highest concern. The calculated deposition fraction of few layer graphene (FLG) with different lateral dimensions ranging from 0.001 to 100 μm in the nasopharyngeal, tracheobronchial, and alveolar regions revealed that there would be substantial deposition of these nanoplatelets throughout the respiratory tract. Findings from several recent studies indicate that graphene and graphene oxide (GO) may induce the acute inflammation and pulmonary fibrosis in mice lungs.

Considering the wide distribution range of graphene sizes in our study, a fraction of graphene likely passed the air-blood barrier and was translocated to liver and spleen. Li et al applied 125I labeled GO to study its distribution in Kunming mice by intratracheal instillation and found that the radioactivity was detected in blood and organs, including liver, spleen and thyroid gland

Nanoparticles may translocate to extrapulmonary organs and may be redistributed to other tissues after deposition in the lung.

Thus, knowledge of graphene biodistribution in mice after inhalation remains a key research gap. FLG may pass through the air-blood barrier into blood and then be delivered to liver and spleen, or enter into the blood via adsorption through the gastrointestinal tract.

https://www.grapheneuses.org/graphene-sensor/ Graphene Sensor Can Detect COVID-19 in 60 Seconds - Graphene Uses

https://www.nsmedicaldevices.com/news/graphene-sensor-covid-19-test/ GLC-GLCM develops graphene-enhanced sensor for rapid Covid-19 test

https://phys.org/news/2020-08-graphene-oxide-based-rapid-infections.html Researchers develop a graphene oxide-based rapid test to detect infections

IN CASE YOU EVER WONDER WHY IT'S INSERTED CLOSE TO YOUR BRAIN AND NOT CLOSE TO YOUR LUNGS:

https://www.sciencedirect.com/science/article/pii/S2666386420301879 Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets

The nasal route represents a means by which nanomaterials can gain access to the brain in exposed individuals.

Highlights

Thin graphene oxide sheets can translocate from the nasal cavity to the brain

Translocation is size dependent, with ultrasmall nanometric sheets translocating the most

Kinetics of graphene oxide accumulation are time dependent and brain-region-specific

After 24 h, besides being present in the olfactory bulbs, trace quantities of us-GO translocated to more distant structures, such as the cortex, striatum, hippocampus, midbrain, cerebellum, and the pons and medulla.

In this previous study, splenic macrophages sequestered GO sheets and mediated their structural degradation over a 9-month period.

In conclusion, our results suggest that following the intranasal administration of aqueously dispersed GO sheets, the materials underwent size-dependent translocation to the brain. The smallest sheet size category (us-GO, 10–550 nm) experienced the greatest translocation and was present in every examined brain region, notably in the olfactory bulb.