15 Comments

Published online July 02, 2014 10.1021/nn5020787

C 2014 American Chemical Society

ABSTRACT The objective of this study was to develop an injectable and biocompatible hydrogel which can efficiently deliver a nanocomplex of graphene oxide (GO) and vascular endothelial growth factor-165 (VEGF) pro-angiogenic gene for myocardial therapy. For the study, an efficient nonviral gene delivery system using polyethylenimine (PEI) functionalized GO nanosheets (fGO) complexed with DNA VEGF was formulated and incorporated in the low-modulus methacrylated gelatin (GelMA) hydrogel to promote controlled and localized gene therapy. It was hypothesized that the fGOVEGF /GelMA nanocomposite hydrogels can efficiently transfect myocardial tissues and induce favorable therapeutic effects without invoking cytotoxic effects.

/nanoparticle-based gene delivery system can be used to develop advanced bioactive hydrogels with tissue-specific functionalities. Such a system can efficiently deliver biotherapeutic molecules in a controlled and localized manner, as well as it can utilize the cell's own machinery for continuous and sustained production of the therapeutic protein, which is not possible with bulk protein delivery methods. 10,11 Studies so far have shown that mammalian viral gene delivery vectors, in combination with hydrogels, can be used for gene delivery applications. 12À15 However, nonviral vectors are clinically more attractive because of their advantageous features including superior biosafety profile, reduced risk of adverse immune reaction and negligible chance of viral gene integration to the host genome thereby zero risk of insertional mutagenesis. 16,17 A major disadvantage of nonviral nanoparticles compared to viral systems is poor transfection efficiency. It has been recently reported that graphene oxide (GO) nanosheets, a precursor of graphene, can be efficiently used to deliver genes efficiently when ionically bonded to cationic polymers such as PEI. PEI is known as a suitable material for gene transfer because it binds strongly to DNA, demonstrates proton sponge effects, and helps in escape of the delivered nucleic acids from endosomal/lysosomal pathways after cell internalization. Low molecular weight branched PEI is also known to have low cytotoxicity and can significantly enhance gene delivery efficiency in combination with GO. 18À21 This is mainly because of its unique delivery features such as suitable water dispersibility, high surface area and aspect ratio, efficient biomolecule loading and effective cell internalization properties.22,23

In this regard, understanding the therapeutic potential of a hydrogel-based GO gene delivery system approach will be beneficial for enabling a range of therapeutic applications. In this study, we have developed a low-modulus GelMA hydrogel, which is a chemically modified form of native gelatin protein. It was selected because of its biocompatibility, biodegradability and ability to support the formation of microvasculature and endothelial cord formation in vitro and in vivo. 24À27 This modified form of denatured collagen has the unique advantages of both natural and synthetic biomaterials.

Expand full comment
author

And it has been implanted in 6 billion people in the world! HEALTHY PEOPLE!!! CHILDREN AND BABIES!!!

Expand full comment

The psychopathy of these scientists beggars the imagination. How can they possibly be oblivious to the potential risks?!?!?!

Expand full comment
Mar 30, 2023·edited Mar 30, 2023

They are not oblivious to risks. They are doing everything in their power in order to enable them. It’s over for this world, humanity and life in general.

Btw: who is this author? What credentials does he have?

Expand full comment

two links provided at beginning of post to the article & paper.

Expand full comment

We the People are facing HORRIFYING TECHNOCRATIC PSYCHOPATHY TO DESTROY HUMANITY AND ALL NATURAL LIFE!

It was NEVER about health! The Powers That Should Not Be were ALWAYS about they want you DEAD or a SLAVE! This is a painful truth to accept but we the people must wake up and fight back!

Supporting this excellent post with a statement and useful links.

We must never lose sight of the larger picture of the vile malignance we are fighting against.

There is an insidious global ruling class plot to enslave all life on earth behind all the madness and suffering inflicted on We the People.

How to fight back against this TOTAL SLAVERY!

RESIST! DO NOT COMPLY! DITCH THE DAMNED 'SMART' PHONES AND THE DAMNED QR CODES AND GO BACK TO LANDLINES OR FLIP PHONES AND USE CASH AS MUCH AS POSSIBLE! INSIST ON CASH! CBDC IS TOTAL SLAVERY!

Other than getting rid of nuclear weapons which I support 100% the rest of the anti-nuclear peace movement and CLIMATE CRISIS propaganda is parroting UN utter GARBAGE, a complete surrender to the ENSLAVEMENT AGENDA by the diabolical despots of Davos - ruling class criminals who lust for total power and control and all of whom should be tried and jailed for life and their malign organizations dismantled: the UN, the WEF, the IMF, the WHO, the BIS, NATO, Blackrock, Vanguard, The Rockefellers, the Rothschilds, The Bilderbergers, the CFR et al.

There is an evil predator globalist technocratic elite agenda of eugenics/depopulation/genocide using bioweapon poison jabs, war, geoengineering, EMF radiation, starvation and economic collapse - THE GREAT RESET/AGENDA 2030/4TH INDUSTRIAL REVOLUTION to get rid of billions of 'useless eaters' and to use nano tech to turn the survivors into ROBOTIZED COMPLIANT SLAVES! WAKE UP AND RESIST! DO NOT COMPLY! These are psychopath megalomaniacs who want to play god by turning all life into digitized metaverse mechanistic synthetic biology to be manipulated by their AI algorithms. A more demonic sickening idea is nearly impossible to imagine!

APPALLED AND HORRIFIED AT INSANE TYRANNICAL PROTOCOLS THAT HAVE NOTHING TO DO WITH HEALTH AND EVERYTHING TO DO WITH TOTALITARIAN CONTROL! REVERSE THIS NOW!

MAKE THE WORLD AND AMERICA 2019 (comparatively speaking), AND FREE AGAIN!

NO, I AM NOT AFRAID OF THE MORONIC SCARIANT SHMARIANT MONKEYSHINES! WAKE UP ALREADY!

TOTALLY CONDEMN BIDEN AND ALL OTHER POLS WHO HAVE NO POWER TO LAWLESSLY ACT LIKE AN EMPEROR OR DICTATOR AND DECREE JAB CROW 'SHOW ME YOUR PAPERS' FASCIST SEGREGATION/DISCRIMINATION/APARTHEID VIOLATIONS OF THE CONSTITUTION, THE NUREMBERG CODES AND EVERY CIVIL RIGHT IMAGINABLE.

NO GREEN NEW DEALS OR BUILD BACK BETTER FROM THE CRIMINAL TECHNOCRAT TYRANTS KLAUS SCHWAB AND HIS CRONIES FROM THE WORLD ECONOMIC FORUM.

STOP THE TERRIBLE TYRANNY OF THE TECHNOCRATS GLOBAL AGENDA OF TOTAL SURVEILLANCE AND CONTROL USING THE VIRUS AS EXCUSE AND PROPAGANDA TOOL!

NO MUZZLING STIFLING MASK MANDATES! NO FORCED VACCINES! END TORTUROUS DEVASTATING LOCK DOWNS NOW! I WANT MY LIFE BACK.

https://wrenchinthegears.com

https://www.stopworldcontrol.com/

https://www.thelastamericanvagabond.com

https://pandemicfacts.info

https://wearehumanwearefree.org/7-days-campaign/

https://sonsoflibertymedia.com/covid-19-roadmap-12-step-plan-to-create-a-totalitarian-new-world-order-were-on-number-8-headed-towards-number-9/

https://questioningcovid.com

https://www.corbettreport.com/interview-1581-james-corbett-breaks-down-the-great-reset/

http://www.stopcp.com/GlobalResetPSYOP/GlobalResetPSYOPMindMap.html

https://everydayconcerned.net/2020/09/04/breaking-major-investigative-report-by-association-of-french-reserve-army-officers-finds-covid-19-pandemic-to-have-a-hidden-agenda-for-global-totalitarianism-nanotech-chipping-of-all-5g-irradia/

Pam Popper: https://makeamericansfreeagain.com

Del Bigtree: https://www.brighteon.com/channels/highwire

https://www.technocracy.news

Naomi Wolf: https://dailyclout.io

www.nojabforme.info

https://www.globalresearch.ca/we-must-awaken-from-corona-coma-reject-great-reset-robotic-technocracy-assert-common-humanity/5745213

Expand full comment

VIRUSES DON'T EXIST!!!

Expand full comment

viruses are fake

Expand full comment

Stunning! Small, effective, deadly!

The hubris to plow full steam ahead in the use of nanotechnology with NO information about long term problems, and the short term medical use is signaling lung, heart, liver etc damage!!

Expand full comment

Checked out your link. Just because it may be possible to use the nano tech to fulfill the wet dream of mortality does it mean it should be done? The ‘plan’ for all this is in the hands of Neanderthals! Who show no enlightened discernment or discriminatory wisdom. Results could be extinction of humans, and various other natural life forms. No humility or respect for creator or creation.

Are we having fun yet !

Expand full comment
author

This is not a new technology. It is no secret why these adverse effects occur.

I do not understand academics who know this research and remain silent.

Expand full comment

I can think of two the reasons for silence 1. To keep their $ life styles

2. To have a seat at the ‘in’ table for the perceived goodies, spot in the bunker, immortality etc.

there are more reasons I’m sure.

Expand full comment
author

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577996/ Carbon nanotubes in COVID-19: A critical review and prospects

Expand full comment